immpress hrp horse antimouse igg (Vector Laboratories)

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Vector Laboratories immpress hrp horse antimouse igg
Immpress Hrp Horse Antimouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horse+antimouse+igg/HRP+Horse+Anti-Mouse+IgG+Antibody+(Peroxidase)/pm42017649-81-79-85
Average 96 stars, based on 1949 article reviews

immpress hrp horse antimouse igg - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: Heterologous expression and purification of the phage lysin-like bacteriocin LysL from Lactococcus lactis LAC460
Article Snippet: Next, the membrane was probed with 1:10 000 diluted in 5% Milk-TBST primary mouse anti-His.H8 (Thermo Scientific) at 4°C overnight with mild agitation. .. It was then incubated with horse antimouse IgG(H + L) Peroxidase (Vector Laboratories, Oxfordshire, UK) at a 1:20 000 dilution in TBST buffer (0.1% Tween 20, 25 mM Tris, 150 mM NaCl, pH 7.5) at room temperature for 1 h. After the last three steps, the membrane underwent three washes, each lasting 5 min, with TBST buffer. .. Protein bands were lastly detected using WesternBright ECL HRP substrate (Advansta, San Jose, CA, USA) with the ChemiDoc MP Imaging System (Bio-Rad Laboratories) according to the producer’s instructions.

Article Title: Isoform‐independent promotion of contractility and proliferation, and suppression of survival by with no lysine/K kinases in prostate stromal cells
Article Snippet: .. Subsequently, membranes were also washed with PBS- T (4 times, each time for 5 min), followed by incubation with secondary biotinylated goat anti- rabbit IgG (BA- 1000) or horse antimouse IgG (BA- 2000) (Vector Laboratories, Burlingame, CA, USA) diluted 1:1500 in PBS- T (containing 5% milk powder), washed again with PBS- T (4 times, each time for 5 min), incubated with avidin and biotinylated horseradish peroxidase (HRP) from the “Vectastain ABC kit” (Vector Laboratories, Burlingame, CA, USA) both diluted 1:800 in PBS and washed again with PBS- T (4 times, each time for 5 min). .. Finally, blots were developed with enhanced chemiluminescence (ECL) using ECL Hyperfilm (GE Healthcare, Freiburg, Germany).

Article Title: Heterologous expression and purification of the phage lysin-like bacteriocin LysL from Lactococcus lactis LAC460.
Article Snippet: .. It was then incubated with horse antimouse IgG(H + L) er oxidase (Vector Labor atories, Oxfordshir e, UK) at a 1:20 000 diution in TBST buffer (0.1% Tween 20, 25 mM Tris, 150 mM NaCl, H 7.5) at room temperature for 1 h. After the last three steps, he membrane underwent three washes, each lasting 5 min, with BST buffer. .. Protein bands were lastly detected using Westernright ECL HRP substr ate (Adv ansta, San J ose , C A, USA) with the hemiDoc MP Imaging System (Bio-Rad Laboratories) according o the producer’s instructions. rotein purification ysL originating from 900 ml of IPTG-induced culture of E. coli CO859 was purified with affinity c hr omatogr a phy using 6 × Hisag as the purification tag b y outsour ced protein purification serice (Protein Service core facility of the Tamper e Univ ersity).

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immpress hrp horse antimouse igg (Vector Laboratories)

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